Purification of the Ivory Shell Toxin

Effect of ivory shell toxin on the parasympathetic ganglia of the dog urinary bladder.

Ivory shell toxin (IST) was first isolated by Hashimoto et al. (1967) from the Japanese ivory shell (Babylonia japonica) which causes sporadically food-poisoning in Japan. Hirayama et al. (1970) found that the toxin is extremely potent in blocking autonomic ganglia. Since the parasympathetic ganglia of the dog urinary bladder have been shown to possess both nicotinic and muscarinic receptors (T...

Studies on the purification of diphtherial toxin.

Purification of tritium-labeled cholera toxin.

Cholera toxin was labeled with tritium by the Wilzbach technique, and highly purified radiolabeled toxin was obtained by Sephadex column chromatography and disc gel electrophoresis. 3H-labeled cholera toxin retained its biological activity and chemical stability and had a specific activity of 405.9 muCi/mumol. The methods utilized in extraction and purification of 3H-labeled toxin may be advant...

The Partial Purification of the Lethal Toxin of Clostridium Histolyticum *

Although several bacterial toxins have been prepared in highly purified form (c.f. Pappenheimer,6 Lamanna et al,,' and Pillemer8), comparatively little effort has been made in this direction with the toxins of the gas gangrene organism C. histolyticurm. Weinberg and Seguinll demonstrated that it could be precipitated with ammonium sulfate, and made the observation that ethanol destroys the toxi...

Rapid and simple purification of lysozyme from the egg shell